This lecture overviews 3D Medical Image Acquisition that has many applications in medical imaging and diagnosis. It covers the following topics in detail: 3D Computed Tomography (including Cone Beam Tomography and Micro-Computed Tomography). 3D Magnetic Resonance Tomography (including Functional MRI Magnetic Resonance Elastography, Diffusion MRI). 3D Ultrasonography. 3D Nuclear Tomography, Single-Photon Emission Tomography (SPECT) Positron Emission Tomography (PET), Magnetic Particle Imaging (MPI). Photoacoustic Tomography (PAT). Non-contact 3D Surface Imaging, notably Structured-light scanning (SLS) and Stereophotogrammetry. 3D Medical Optical Imaging, including Diffuse Optical Tomography, Optical Coherence Tomography and Confocal Laser Scanning Microscopy (CLSM).