This lecture overviews 2D Medical Image Acquisition that has many applications in Medical diagnosis and treatment, as well as in Biology, Biomedical Engineering and Dentistry. It covers the following topics in detail: Radiography, Conventional Radiography (X-Ray), Fluoroscopy. Ultrasonography. Nuclear Scan, Scintigraphy. Elastography. Photoacoustic Imaging, Photoacoustic Microscopy (PAM).